Nutritional Sciences

    College of Agricultural & Life Sciences

    UNIVERSITY of WISCONSIN-MADISON UW-MADISON

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    Kidney deletions of Cyp27b1 fail to reduce serum 1,25(OH)2D3

    The Journal of Steroid Biochemistry and Molecular Biology

    • Seong Min Lee a, Shannon R. Cichanski a, Nicolas G. Pintozzi a, Martin Kaufmann bc, Glenville Jones b, Mark B. Meyer a
    • •Cyp27b1 was deleted from the developing kidney by using the Six2-cre and Pax8-cre mice crossed with the Cyp27b1fl/fl mouse.
    • •Pax8-cre/Cyp27b1fl/fl mice have elevations in PTH and decreases in bone mineral density consistent with perturbation of the vitamin D system.
    • •CYP24A1 enzymatic activity and Cyp24a1 mRNA expression were reduced in the Pax8-cre/Cyp27b1fl/fl mice more so than the Six2-cre/Cyp27b1fl/fl.
    • •Despite the apparent loss of Cyp27b1 mRNA expression and reductions in Cyp24a1 mRNA expression, including altered vitamin D metabolic profiles, the levels of 1,25(OH)2D3 were unchanged in either deletion model.
    • https://www.sciencedirect.com/science/article/pii/S0960076025000627?dgcid=author

    Abstract

    Vitamin D metabolism is controlled through the kidney mitochondrial P450 enzymes 1α-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24A1) that activate and degrade the endocrine vitamin D hormone (1,25(OH)2D3), respectively. We recently demonstrated that extrarenal cells can make 1,25(OH)2D3 with adequate vitamin D supplementation by targeted mass spectrometry imaging in our Cyp27b1 kidney enhancer deletion mouse model that lacks circulating 1,25(OH)2D3 (M1/M21-DIKO mouse). Based on these observations, we selectively deleted Cyp27b1 (Cyp27b1fl/fl) from the mouse kidney using the Six2- and Pax8-cre drivers that target tubule and nephron development to see if we could recapitulate the remarkable phenotype of the M1/M21-DIKO mice. While Six2-cre/Cyp27b1fl/fl mice had a mild phenotype, Pax8-cre/Cyp27b1fl/fl mice had a marked elevation of parathyroid hormone and a reduction in bone mineral density. The vitamin D metabolic profile in the Pax8-cre/Cyp27b1fl/fl clearly indicated a dysfunction in the CYP24A1 enzyme with reductions in 24,25(OH)2D3 and 25(OH)D3-26,23-lactone with an accompanying elevation of 25(OH)D3. However, despite these compensatory reductions in CYP24A1 derived metabolites and apparent deletion of Cyp27b1 in the kidney, the 1,25(OH)2D3 levels were not changed from wildtype in either mouse. Like 24,25(OH)2D3, the 1,24,25(OH)3D3 levels were also reduced. These data highlight the robust homeostatic mechanisms to salvage 1,25(OH)2D3, point towards potential compensatory mechanisms of 1,25(OH)2D3 production from non-kidney tissues, and reinforce the utility of the M1/M21-DIKO model as a non-global deletion of Cyp27b1 with reductions in serum 1,25(OH)2D3 to be used to understand the complexity of vitamin D metabolism in health and inflammatory disease.

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