Phone
608-890-2640Website
View WebsiteOffice Location
1605 Linden Drive,
Room A203B
Madison, WI, 53706
Education
Education B.S., Biotechnology, Noordelijke Hogeschool Leeuwarden, The Netherlands (1999)
M.S., Biology, Leiden University, The Netherlands (2002)
Ph.D., Microbiology, University College Cork, Ireland (2007)
- Genome editing of phylogenetically distinct bacteria using portable retron-mediated recombineering
- Synthesis of potential prebiotic oligosaccharides with diverse glycosidic linkages from corn stover via simultaneous hydrolysis and glycosylation
- A secondary metabolite of Limosilactobacillusreuteri R2lc drives strain-specific pathology in a spontaneous mouse model of multiple sclerosis
- Allosteric regulation of pyruvate kinase enables efficient and robust gluconeogenesis by preventing metabolic conflicts and carbon overflow
- Development of a <em>Limosilactobacillus reuteri</em> therapeutic delivery platform with reduced colonization potential
- Allosteric Regulation of Pyruvate Kinase Enables Efficient and Robust Gluconeogenesis by Preventing Metabolic Conflicts and Carbon Overflow
- Genetically Engineered Probiotic <em>Limosilactobacillus reuteri</em> Releasing IL-22 (LR-IL-22) Modifies the Tumor Microenvironment, Enabling Irradiation in Ovarian Cancer
- Selective utilization of gluco-oligosaccharides by lactobacilli: A mechanism study revealing the impact of glycosidic linkages and degree of polymerization on their utilization
- Bioluminescent monitoring of recombinant lactic acid bacteria and their products
- Probiotic-Derived Ecto-5'-Nucleotidase Produces Anti-Inflammatory Adenosine Metabolites in Treg-Deficient Scurfy Mice
We work at the interface of industry and medicine. While we have completed projects with various lactic acid bacteria, most of our research focuses on Limosilactobacillus reuteri, until recently known as Lactobacillus reuteri. L. reuteri is an important gut symbiont, and select strains have demonstrated health-promoting, i.e., probiotic, properties. The ultimate goal of our research program is to understand probiotic mechanisms towards the development of next-generation probiotics.
For many years, we have invested in developing genetic tools for use in L. reuteri and other lactic acid bacteria. As a consequence, our laboratory is at the forefront of lactic acid bacteria genome editing. These genome editing tools place us in the exciting position to pursue research in the following areas:
(1) to unravel the molecular underpinnings by which probiotic bacteria interact with the vertebrate host;
(2) to unravel the tripartite interplay between diet, a probiotic, and its bacteriophages;
(3) to develop probiotic bacteria as therapeutic delivery vehicles.
We’re grateful for our current and past funding, which, combined with great collaborators, made it possible to build a dynamic team of staff and students to drive our research.
If you are interested in learning more about our work, please don’t hesitate to contact us.